FIGURE 4.

Radiolabeled glucose uptake in hairy roots. Five root fragments (30-day-old) from A4, pBin and VvSWEET4_OX lines were collected, and placed for 60 min (2 times) in equilibration buffer. Total glucose uptake of roots was measured after 15 min of incubation with 10 mM D-[¹⁴C]glucose. To measure glucose diffusion, CCCP (20 μM) was added into the equilibration buffer 10 min before addition of incubation buffer. Active glucose uptake results from the difference between total uptake and diffusion. Data are the mean ± SE of four independent replicates. Means with different letters are significantly different at p ≤ 0.05 (Tukey Contrasts).