Figure 3.

Primary cortical neuron cell viability. Administration of Hyperbaric oxygen therapy (HBOT) precondition occurred 24 h prior to injury onset. Primary rat neuronal cells (PRNCs) cocultured with astrocytes were subjected to HBOT for 90 min in the preconditioning group. After the insult was introduced to the PRNCs cocultured with astrocytes for 24 h, the cell viability was analyzed by Calcein‐AM staining. Cell counting was performed and standardized per 1 cm². Intensity of signal was also calculated as described in the materials and methods. *P < 0.05, **P < 0.01, ***P < 0.001. The scale bar corresponds to 20 μm