FIGURE 4.

Effect of combination treatment with XSEC and EE on the endogenous neurogenesis in MCAO rats. (A,B) Typical photographs of double immunofluorescent staining for Map-2/Ki67 and GFAP/Ki67 in the peri-infarct cortex and striatum (white arrows indicated co-located cells). (C) Magnified images of co-expressed cells of Map-2/Ki67 ⁺ and GFAP/Ki67⁺ (arrowheads: Map-2/Ki67⁺ co-expressed cell; arrows: GFAP/Ki67⁺ co-expressed cell). (D) Schematic diagram of the ischemic brain. The yellow square represents peri-ischemic cortex region and green for striatum that wasdissected for immunofluorescence. (E–G) Quantitative data of the number of Ki67⁺ cells per mm² and the percentage of Map-2/Ki67 ⁺ and GFAP/Ki67⁺ co-expression cells divide by total Ki67⁺ cells numbers in the peri-infarct cortex and striatum. Data were presented as mean ± SEM, n = 4, ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001 vs. vehicle; ^∧P < 0.05, ^∧∧P < 0.01 vs. XSEC+EE.