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. 2019 Apr 11;7(2):42. doi: 10.3390/dj7020042

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Biofilm 3D reconstruction of confocal laser scanning microscopy. Computerized 3D reconstruction (X, Y, and Z axis) of the biofilm layers (in µm), recorded using CLSM and generated by the zen2009 software. (a) Control S. mutans and C. albicans cells and EPS. (b) S. mutans cells only. (c) S. mutans EPS only. (d) Candida cells only. (e) Candida EPS only. (f) Treated S. mutans and C. albicans biofilm. (g) S. mutans cells only. (h) S. mutans EPS only. (i) Candida cells only. (j) Candida EPS only.