Figure 3.

The proposed secondary structure of the subgroup A to E ASLV envelope glycoproteins. Shown are the disulfide bonds determined using a His-tagged, secreted form of the RCASBP(A) envelope glycoprotein expressed using chicken DF-1 cells and purified. The bonds to C11 and C12 (indicated with a box) could not be assigned further. The free cysteine is labeled C2. The N-linked glycosylation sites actually containing carbohydrate are marked in blue. The unglycosylated sites are underlined, N8 and N13. For reference, the hypervariable regions are marked: hr1 in green boxes; hr2 in an orange box; and vr3 in a pink box.