Skip to main content
. 2019 Jun 25;24(12):2344. doi: 10.3390/molecules24122344

Figure 2.

Figure 2

Figure 2

Effect of KWM-EO on cell-cycle and apoptosis in PDV cells. (A) PDV cells were exposed to vehicle or 75 μg/mL KWM-EO in the presence or absence of 0.5 μM PLX4032 for 12 or 24 h, then the cell-cycle was analyzed by flow cytometry. (B) PDV cells were treated with vehicle or 75 μg/mL KWM-EO in the presence or absence of 0.5 μM PLX4032 for 24 h. Cells were then stained with annexin V and propidium iodide, and the cell apoptosis was detected by flow cytometry. (C) PDV cells were treated with 75 μg/mL KWM-EO in the presence or absence of 0.5 μM PLX4032 for 24 h before lysis. The cell lysates were subjected to Western blotting against cell-cycle-related proteins, including p-cdc2 (Thr161), p-cdc25C, and cyclin B1. (D) The expression level of apoptosis-related proteins in PDV cells treated with 75 μg/mL KWM-EO in the presence or absence of 0.5 μM PLX4032 for 6 h was examined by Western blotting against PARP-1 and caspase 3. (E) Western blotting analysis of MAPK signaling-related proteins (p-ERK, ERK, p-MEK, MEK) in PDV cells treated with 75 μg/mL KWM-EO in the presence or absence of 0.5 μM PLX4032 for 24 h. Actin was used as an internal control in the experiment. Vehicle controls (C) were obtained from cells treated with 0.5% DMSO. The data are representative of three independent experiments and are expressed as mean ± SD. N.S. means non significance; P*** < 0.05 compared to vehicle control; P## < 0.01, P### < 0.001 compared to PLX4032-treated group (ANOVA).