Table 1.
Antibody Combinations | Group | +/− (%) | +/+ (%) | −/+ (%) |
---|---|---|---|---|
CD3/CD45R | VHF | 9.87 ± 0.89 | 0.81 ± 0.16 | 36.93 ± 5.66 |
3 | 10.72 ± 1.91 | 0.93 ± 0.12 | 35.78 ± 2.17 | |
30 | 9.18 ± 0.42 | 0.86 ± 0.06 | 34.27 ± 1.29 | |
150 | 12.04 ± 1.58 | 1.17 ± 0.17 | 37.40 ± 1.93 | |
300 | 9.96 ± 0.59 | 1.18 ± 0.11 | 36.24 ± 1.36 | |
CD4/CD8 | VHF | 7.44 ± 1.17 | 1.82 ± 0.10 | 9.26 ± 1.29 |
3 | 7.70 ± 1.31 | 1.62 ± 0.27 | 8.39 ± 0.83 | |
30 | 6.38 ± 0.47 | 1.94 ± 0.13 | 10.54 ± 0.87 | |
150 | 8.53 ± 1.17 | 2.39 ± 0.11 | 11.47 ± 0.37 | |
300 | 6.82 ± 0.34 | 2.42 ± 0.20 | 12.64 ± 1.46 | |
CD4/CD25 | VHF | 7.68 ± 0.98 | 0.97 ± 0.12 | 0.36 ± 0.07 |
3 | 7.73 ± 1.33 | 1.11 ± 0.12 | 0.48 ± 0.05 | |
30 | 6.83 ± 0.43 | 0.91 ± 0.09 | 0.40 ± 0.03 | |
150 | 8.87 ± 1.22 | 1.36 ± 0.02 | 0.61 ± 0.09 * | |
300 | 7.63 ± 0.46 | 1.00 ± 0.93 | 0.50 ± 0.04 | |
CD8/CD25 | VHF | 5.71 ± 0.61 | 0.37 ± 0.10 | 0.85 ± 0.11 |
3 | 5.66 ± 0.80 | 0.39 ± 0.05 | 1.06 ± 0.11 | |
30 | 6.85 ± 0.62 | 0.32 ± 0.03 | 0.87 ± 0.08 | |
150 | 8.14 ± 0.44 | 0.49 ± 0.05 | 1.36 ± 0.09 * | |
300 | 8.78 ± 1.03 | 0.49 ± 0.05 | 0.86 ± 0.06 | |
CD24/CD5 | VHF | 67.40 ± 1.23 | 4.50 ± 0.03 | 16.47 ± 0.73 |
3 | 63.17 ± 2.60 | 4.18 ± 0.31 | 19.77 ± 3.02 | |
30 | 66.95 ± 1.05 | 4.35 ± 0.36 | 16.08 ± 0.61 | |
150 | 63.37 ± 2.05 | 4.36 ± 0.35 | 19.50 ± 2.85 | |
300 | 66.42 ± 1.50 | 4.47 ± 0.24 | 16.32 ± 1.09 | |
CD40/CD44 | VHF | 32.77 ± 2.35 | 3.83 ± 0.64 | 2.61 ± 0.59 |
3 | 33.50 ± 2.68 | 3.89 ± 0.45 | 2.61 ± 0.28 | |
30 | 30.32 ± 1.58 | 3.67 ± 0.27 | 3.00 ± 0.13 | |
150 | 34.23 ± 2.95 | 3.59 ± 0.87 | 2.51 ± 0.55 | |
300 | 32.30 ± 1.60 | 2.61 ± 0.24 | 2.34 ± 0.20 | |
B220/CD40L | VHF | 28.53 ± 5.97 | 0.59 ± 0.11 | 1.66 ± 0.16 |
3 | 33.10 ± 2.83 | 0.59 ± 0.08 | 1.62 ± 0.20 | |
30 | 29.97 ± 2.67 | 0.53 ± 0.09 | 1.83 ± 0.35 | |
150 | 34.10 ± 3.91 | 0.58 ± 0.11 | 1.48 ± 0.21 | |
300 | 29.52 ± 1.91 | 0.42 ± 0.07 | 1.21 ± 0.15 | |
Mac3/CD45R | VHF | 5.57 ± 0.50 | 7.15 ± 1.29 | 29.33 ± 4.63 |
3 | 5.57 ± 0.27 | 8.32 ± 0.68 | 27.27 ± 1.76 | |
30 | 5.70 ± 0.32 | 7.94 ± 0.88 | 25.87 ± 0.70 | |
150 | 6.44 ± 0.67 | 11.10 ± 0.71 | 26.13 ± 1.47 | |
300 | 5.65 ± 0.67 | 10.32 ± 1.13 | 25.58 ± 0.76 |
* p < 0.05 as compared to the vehicle female (VHF) control group. The values are presented as mean ± standard error of the mean (SEM). N = 3 for the VHF and 150 μg/kg dose groups; N = 6 for the 3 and 30 μg/kg dose groups; N = 5 for the 300 μg/kg dose group.