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. 2019 Jun 15;24(12):2239. doi: 10.3390/molecules24122239

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Determination of the IC₅₀ inhibitory concentration of osmanicin against elastase (A) and cell survival after osmanicin treatment (B). (A) The half maximal inhibitory concentration (IC₅₀) of osmanicin against elastase was calculated by plotting the graph between the different concentrations used (1 μM to 5 μM) and the % inhibition of elastase activity. Data are presented as mean ± SD (n ≥ 3). (B) Relative (%) survival (MTT assay) of BJ fibroblasts exposed to the indicated concentrations of osmanicin for 24 h. Data are presented as mean ± SD (n ≥ 3).