Figure 6.

Kinetic evaluation of SEAP activity in supernatants derived from LPS-treated HEK-Blue hTLR4 cells. Cells at an initial density of 1 x 10⁵/well were cultured overnight with 8 ηM LPS (squares), 8 ηM LPS plus 20 uM Bv (diamonds), or media alone (triangles). Since it had been shown in Figure 5 that Bv alone was non-stimulatory and Bv inhibition of baseline was insignificant, Bv alone treatment was not reiterated here. The 8 ηM concentration of LPS used here corresponds to 0.8 µg/mL based upon a molecular weight of 100,000 (Sigma). A 60 min SEAP time course of substrate conversion was evaluated to show SEAP velocities over time (Slope LPS + Bv = 0.0065, R² = 0.997; Slope LPS = 0.0085, R² = 0.999; No Treatment Slope = 0.0021, R² = 0.989). ANOVA measurement of significance between LPS and LPS + Bv is designated by an asterisk (*; where p < 0.05).