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. 2019 Jun 21;24(12):2295. doi: 10.3390/molecules24122295

Figure 3.

Figure 3

Evaluation of ICG intake in vivo. (a) The ICG signal was observed using IVIS at 4, 24, 48, and 72 h after injecting ICG to NTCP- and OATP1B3-expressing tumor-bearing mice. The tumor on the left hind leg was a controlled tumor, and on the right was an NTCP-expressing tumor at the top and an OATP1B3-expressing tumor at the bottom. (b) The relationship between tumor size and the total efficiency of ICG at 48 h after injecting ICG to NTCP- and OATP1B3-expressing tumor-bearing mice. (c) After the ICG signal was normalized based on control tumor size to obtain relative ICG intensity, we compared the relative ICG intensities in the control and NTCP- and OATP1B3-expressing tumor cells at 4, 24, 48, and 72 h after injecting ICG into the NTCP- and OATP1B3-expressing tumor-bearing mice. N = 3 and N = 4 for NTCP and OATP1B3 tumor-bearing mice, respectively. C: control tumor. N: NTCP-expressing tumor. O: OATP1B3-expressing tumor. Error bars indicate the SEM. * p < 0.05, # p < 0.01, & p < 0.001. *, #, & in purple and green color indicate the control compared with OATP1B3- and NTCP-expressing tumor cells, respectively. * in red color indicates the NTCP-expressing tumor cells compared with OATP1B3-expressing tumor cells. Ctrl: control tumor cells.