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. 2019 Jun 2;11(6):507. doi: 10.3390/v11060507

Figure 2.

Figure 2

Morphology of AEB VLPs and their parental viruses. Rate velocity centrifugation of (A) NL4-3 virions, (B) AEB VLPs, and (C) 93TH253.3 virions was performed. A sample of the VLP concentrate prior to centrifugation (In), the uppermost iodixanol-free fraction of buffer created upon application of sample to the gradient (S), and gradient fractions of increasing density (1–12) were resolved by 6–18% SDS-PAGE before Western blotting using anti-gp120 (DV-012, upper panel) and anti-p24 (BC1071, lower panel). For all panels, protein sizes were indicated by Spectra Multicolor Broad Range Protein Ladder (Thermo Scientific) and are shown on the left. For (A) note the absence of an anti-gp120 upper panel due to 93TH253.3 not expressing a functional Env. Representative Western blots are shown. HEK 293T cells transfected with (D) pCMV-EGFP, (E) pNL4-3, (F) pCMV-AEB, and (G) p93TH253.3 were imaged by transmission electron microscopy (TEM). Representative images are shown for each sample. White scale bar represents 200 nm.