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Determination of anti-BVDV mucosal sIgA antibody in intestinal mucus (A) and feces (C) samples collected from the immunized mice at different time points after immunization by ELISA using BVDV as the coating antigen, and BVDV-neutralizing activity of the intestinal mucosal sIgA antibody (B) collected on days 42 after the primary immunization. Data is represented as mean ± standard error value of each group. “*” means p < 0.05, “**” means p < 0.01.
