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. 2019 Jun 25;11(6):575. doi: 10.3390/v11060575

Table 1.

Primers used in this study.

Primers Sequence (5′–3′) Production Size
BVDV-E2-F CGAGCTCaATGCTCCCAGCCTGTAAACC 1122 bp
BVDV-E2-R TTTGGGCCCaTTAACCTAAGGTCGTTTGTTCTGAT
E2-DCpep GGGCCCa TGGACGTTGTGGAGTTGAATGATATGATGGATAAAAbTGAGCCACCGCCACCcTTAACCTAAGGTCGTTTGTTC
BVDV-F GGTAGCAACAGTGGTGAG 221 bp
BVDV-R GTAGCAATACAGTGGGCC

a. Restriction enzyme sites used for cloning (underline); b. DCpep (in bold); c. Flexible amino acid (in italic).