Table 1.
Primers | Sequence (5′–3′) | Production Size |
---|---|---|
BVDV-E2-F | CGAGCTCaATGCTCCCAGCCTGTAAACC | 1122 bp |
BVDV-E2-R | TTTGGGCCCaTTAACCTAAGGTCGTTTGTTCTGAT | |
E2-DCpep | GGGCCCa TGGACGTTGTGGAGTTGAATGATATGATGGATAAAAbTGAGCCACCGCCACCcTTAACCTAAGGTCGTTTGTTC | |
BVDV-F | GGTAGCAACAGTGGTGAG | 221 bp |
BVDV-R | GTAGCAATACAGTGGGCC |
a. Restriction enzyme sites used for cloning (underline); b. DCpep (in bold); c. Flexible amino acid (in italic).