Table 8.

Primers used for PCR.

Gene	Primer	Primer Sequence (5′-3′)	Annealing Temperature (°C)	PCR Product Size (bp)	Reference
uspA	uspA-F	CCGATACGCTGCCAATCAGT	64	884	[47]
	uspA-R	ACGCAGACCGTAGGCCAGAT
eae	eae-F	CCCGAATTCGGCACAAGCATAAGC	57	881	[19]
	eae-R	CCCGGATCCGTCTCGCCAGTATTCG
aaiC	aaiC-F	ATTGTCCTCAGGCATTTCAC	57	215	[19]
	aaiC-R	ACGACACCCCTGATAAACAA
aat	aat-F	CTGGCGAAAGACTGTATCAT	57	650	[19]
	aat-R	CAATGTATAGAAATCCGCTGTT
stx1	stx1-F	CACAATCAGGCGTCGCCAGCGCACTTGCT	64 *	606	[19]
	stx1-R	TGTTGCAGGGATCAGTGGTACGGGGATGC
stx2	stx2-F	CCACATCGGTGTCTGTTATTAACCACACC	64 *	372	[19]
	stx2-R	GCAGAACTGCTCTGGATGCATCTCTGGTC
ipaH	Shig-F	TGGAAAAACTCAGTGCCTCT	55	424	[19]
	Shig-R	CCAGTCCGTAAATTCATTCT
blaKPC	KPC-Fm	CGTCTAGTTCTGCTGTCTTG	52	798	[49]
	KPC-Rm	CTTGTCATCCTTGTTAGGCG
blaNDM	NDM-F	GGTTTGGCGATCTGGTTTTC	52	621	[49]
	NDM-R	CGGAATGGCTCATCACGATC
blaVIM	VIM-F	GATGGTGTTTGGTCGCATA	52	390	[49]
	VIM-R	CGAATGCGCAGCACCAG
blaTEM	TEM-F	ACATGGGGGATCATGTAACT	52	421	[50]
	TEM-R	GACAGTTACAATGCTTACT
blaSHV	SHV-F	ATGCGTTATATTCGCCTGTG	56	859	[50]
	SHV-R	AGCGTTGCCAGTGCTCGATG
blaCTX	CTX-MU1	ATGTGCAGYACCAGTAARGT	50	593	[50]
	CTX-MU2	TGGGTRAARTARGTSACCAGT

Notes: * PCR involved 40 cycles having initial annealing at 64 °C for two cycles followed by eight cycles having a 2 °C decrease in annealing temperature after every two cycles.