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Plasmid DNA supercoiling assay. The plasmid DNA supercoiling activity was carried out for full-length forms of both AtNRP1 and AtNRP2. The samples were run on 1% agarose gel and stained with ethidium bromide. Relaxed and supercoiled forms of plasmids are labeled in the figure. Topoisomers are the plasmid DNA bands corresponding to different degrees of supercoiling, due to the effect of histone chaperones.
