Table 2. CD Tm Values of RV G4s in the Absence and Presence of G4 Ligands 1 and 2a.
|
Tm (°C) |
ΔTm (°C) |
|||||
|---|---|---|---|---|---|---|
| — | 1 | 2 | 1 | 2 | ||
| beta-RVs | RV1 | 48.1 ± 0.9 | 68.9 ± 0.2 | 60.6 ± 0.8 | 20.8 | 12.5 |
| RV2 | ND | ND | ND | |||
| RV3 | ND | ND | ND | |||
| RV4 | 67.1 ± 1.2 | >90 | >90 | >22.9 | >22.9 | |
| RV5 | 48.0 ± 1.9 | 68.9 ± 3.1 | 85.9 ± 1.2 | 20.9 | 37.9 | |
| ND | ND | 62.3 ± 1.1 | ND | ND | ||
| RV6 | ND | ND | ND | |||
| RV7 | 63.9 ± 0.8 | 75.8 ± 0.9 | >90 | 11.9 | >26.1 | |
| RV8 | ND | ND | ND | |||
| delta-RVs | RV9 | 65.1 ± 0.3 | >90 | >90 | >24.9 | >24.9 |
| 64.9 ± 0.3 | >90 | >90 | >25.1 | >25.1 | ||
| RV10 | 66.4 ± 1.3 | 83.8 ± 2.1 | >90 | 17.4 | >20.6 | |
| 48.9 ± 0.8 | 72.1 ± 0.9 | 70.3 ± 2.5 | 23.2 | 24.4 | ||
| RV11 | 61.4 ± 0.3 | 79.2 ± 0.7 | ND | 14.6 | ND | |
| 56.6 ± 2.1 | 69.0 ± 3.8 | 63.4 ± 0.3 | 12.4 | 6.8 | ||
| RV12 | 63.1 ± 0.4 | ND | ND | ND | ND | |
| 63.3 ± 0.4 | 66.3 ± 0.1 | 66.9 ± 0.8 | 3.2 | 3.8 | ||
| RV13 | ND | ND | ND | |||
| RV14 | 65.5 ± 0.8 | >90 | >90 | >24.5 | >24.5 | |
| gamma-RVs | RV15 | 55.4 ± 0.1 | >90 | >90 | >34.6 | >34.6 |
| ND | 67.0 ± 0.1 | 62.1 ± 2.6 | ND | ND | ||
| RV16 | ND | ND | ND | ND | ND | |
| 53.3 ± 1.4 | 63.4 ± 1.0 | 68.5 ± 2.3 | 10.1 | 15.2 | ||
| RV17 | 52.3 ± 0.8 | 86.7 ± 1.0 | 57.0 ± 3.4 | 33.7 | 4.7 | |
| RV18 | 59.9 ± 0.4 | 76.5 ± 0.1 | 70.6 ± 1.0 | 16.6 | 10.7 | |
| RV19 | 66.8 ± 0.1 | 77.6 ± 0.6 | 85.1 ± 0.1 | 10.8 | 18.3 | |
| RV20 | ND | ND | ND | |||
| RV21 | 56.8 ± 0.1 | ND | 65.6 ± 1.8 | ND | 8.8 | |
| 56.1 ± 0.1 | 60.0 ± 2.4 | 69.6 ± 2.0 | 3.9 | 13.5 | ||
| RV22 | 54.2 ± 0.8 | ND | ND | ND | ND | |
| 54.7 ± 0.6 | 64.9 ± 2.9 | 75.9 ± 3.9 | 10.2 | 21.2 | ||
| RV23 | 56.9 ± 2.7 | 83.8 ± 3.9 | 81.4 ± 2.2 | 25 | 22.6 | |
| RV24 | 41.2 ± 0.2 | 50.8 ± 0.1 | 43.9 ± 3.0 | 9.6 | 2.7 | |
| RV25 | 53.4 ± 0.1 | >90 | >90 | >35.6 | >35.6 | |
| RV26 | 73.6 ± 0.6 | >90 | >90 | >16.4 | >16.4 | |
| RV27 | ND | ND | ND | |||
| RV28 | >90 | >90 | >90 | ND | ND | |
| ND | 58.5 ± 4.5 | 71.0 ± 0.5 | ||||
| RV29 | ND | ND | ND | ND | ND | |
| 53.3 ± 1.4 | 63.4 ± 1.0 | 68.5 ± 2.3 | 10.1 | 15.2 | ||
a
Data are reported as mean values ± SD from at least two independent experiments. In cases of double transitions, Tm values calculated at λ = 260 nm (first value) and 290 nm (second value) are shown.