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Caspase-3 assay of barbatic acid (21). A549 cells were seeded and treated with 1 and 2 µM of 21. Subsequently, the cells were harvested, and lysates were subjected to cellular caspase 3 analysis. As a positive control doxorubicin was employed. UT represents untreated cells and D is DMSO, vehicle control. Values are mean ± SD, the assay was repeated two times in triplicates (n = 6). * p < 0.05 vs. control is treated as statistically significant.
