Figure 3.

Neutrophils Drive DNT_αβ Type 1 Polarization

(A) Expression of IFNγ by tumor-infiltrating T cells stimulated ex vivo by PMA plus ionomycin.

(B) Representative dot plot showing Rorγt, and T-bet expression in UTC_αβ from Csf3r^+/+ and Csf3r^−/− tumors.

(C and D) Quantification of Eomes, Rorγt, and T-bet expression in UTC_αβ from Csf3r^+/+ and Csf3r^−/− tumors (C) and in Csf3r^−/− mice after adoptive transfer of neutrophils (D).

(E) Expression of Eomes, Rorγt, and T-bet in UTC_αβ infiltrating the 3-MCA injection site (10 days after administration of 3-MCA).

(F) Quantification of iNKT, MAIT, and DNT_αβ frequencies among sarcoma-infiltrating CD45⁺ cells.

(G) Polarization of tumor-associated DNT_αβ cells after neutrophil adoptive transfer.

(D and G) 3 × 10⁶ neutrophils were transferred i.v. once a week starting from the first day the tumor was palpable. (E) 3 × 10⁶ neutrophils were transferred i.v. at days −1, 0, 1, and 9 with respect to 3-MCA administration. (A and C–G) Data are mean ± SEM. ^∗p ≤ 0.05, ^∗∗p ≤ 0.01, ^∗∗∗p ≤ 0.001. (A, C, and F) Two-tailed multiple Student’s t tests. (D) One-way ANOVA. (E and G) Kruskal-Wallis test with Dunn’s multiple comparison test.

See also Figures S3 and S4.