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Anti-ZIKBeHMR-2 antibodies recognize the E protein from ZIKBeHMR-1. Vero cells were infected 24 h with ZIKBeHMR-1 or ZIKBeHMR-1 at m.o.i. of 1 or mock-infected (m.i.) and then lysed with RIPA lysis buffer. Immunoblot assay on cell lysates was performed with pooled immune sera of mice that received two doses of 5 log PFU of ZIKBeHMR-2 (ZIKBeHMR-2 antisera) with an interval of 6 weeks. The expression of E was verified with anti-E mAb 4G2 (anti-E Ab).
