Table 1.
Cytokine and chemokine production by F. alocis-stimulated human neutrophils.
| Cytokines | Basal | Non-op-F. alocis | Op-F. alocis |
|---|---|---|---|
| IL-1β (pg/0.5 × 106 cells) | 1.30 ± 0.736 | 5.125 ± 0.417 ** | 5.464 ± 0.720 ** |
| TNFα (pg/0.5 × 106 cells) | 6.448 ± 1.302 | 46.759 ± 6.382 ** | 35.514 ± 7.401 * |
| IL-1ra (pg/2 × 106 cells) | 38.948 ± 7.785 | 194.527 ± 24.179 *** | 138.451 ± 7.678 ** |
| CXCL Chemokines | |||
| CXCL1 (ng/2 × 106 cells) | 0.004 ± 0.002 | 0.478 ± 0.078 *** | 0.445 ± 0.073 *** |
| CXCL8 (ng/0.1 × 106 cells) | 0.596 ± 0.214 | 4.492 ± 1.166 * | 3.818 ± 0.849 * |
| CCL Chemokines | |||
| CCL3 (pg/2 × 106 cells) | 25.087 ± 10.922 | 108.511 ± 16.580 | 232.526 ± 80.041 * |
| CCL4 (pg/2 × 106 cells) | 133.315 ± 43.227 | 639.611 ± 104.467 | 709.178 ± 203.417 * |
Note. Human neutrophils were stimulated with non-opsonized (Non-op) or opsonized (Op) F. alocis (MOI 10:1) for 24 h. Cell/bacteria free supernatant were collected and cytokine and chemokine release measured by ELISA (IL-1β, TNFα, CXCL8) or Milliplex Luminex (IL-1ra, CXCL1, CCL3, CCL4). Data are presented as mean ± SEM. IL-1β, CCL3 n = 4; CXCL8, TNFα, CCL4 n = 5; IL-1ra n = 6; CXCL1 n = 7. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to basal.