Table 2.
Gene specific primers.
| Genes | Sequence | |
|---|---|---|
| TNF-α | Forward | 5’CAGCCTCTTCTCCTTCCTGAT3’ |
| Reverse | 5’GCCAGAGGGCTGATTAGAGA3’ | |
| IL-1ra | Forward | 5’AACTAGTTGCTGGATACTTGCA3’ |
| Reverse | 5’CCAGACTTGACACAGGACAG3’ | |
| IL-1b | Forward | 5’TACCTGTCCTGCGTGTTGAA3’ |
| Reverse | 5’TCTTTGGGTAATTTTTGGGATCT3’ | |
| CXCL1 | Forward | 5’AACCGAAGTCATAGCCACAC3’ |
| Reverse | 5’CCTCCCTTCTGGTCAGTTG3’ | |
| CXCL2 | Forward | 5’AACCGAAGTCATAGCCACAC3’ |
| Reverse | 5’CTTCTGGTCAGTTGGATTTGC3’ | |
| CXCL3 | Forward | 5’AAGTGTGAATGTAAGGTCCCC3’ |
| Reverse | 5’GTGCTCCCCTTGTTCAGTATC3’ | |
| CXCL8 | Forward | 5’GAGCACTCCATAAGGCACAAA3’ |
| Reverse | 5’ATGGTTCCTTCCGGTGGT3’ | |
| CCL3 | Forward | 5’CGGCAGATTCCACAGAATTTC3’ |
| Reverse | 5’AGGTCGCTGACATATTTCTGG3’ | |
| CCL4 | Forward | 5’TCCTCGCAACTTTGTGGTAG3’ |
| Reverse | 5’TTCAGTTCCAGGTCATACACG3’ | |
| GAPDH | Forward | 5’CTTTGGTATCGTGGAAGGACTC3’ |
| Reverse | 5’GTAGAGGCAGGGATGATGTTC3’ |