Table 3.
Differential expression of glutathione (GST) and glycosyltransferase (GT) genes via RNA-Seq analysis of glyphosate-resistant (GR) and -sensitive (GS) C. bonariensis biotypes in response to glyphosate treatment up to 288 h after treatment. The transcriptional fold change data for the GR and GS biotypes are each expressed as the ratio of the ABC transporter gene expression from glyphosate treated to untreated individuals. Therefore, a value of 1 means the gene was neither induced nor repressed. The rightmost column represents the ratio of ratios; i.e., the magnitude of effect of being glyphosate-resistant.
| No | Contig ID | UniProt ID | Functional Annotation | Putative Localization | Transcription Fold Change | Ratio GR/GS | |
|---|---|---|---|---|---|---|---|
| GR | GS | ||||||
| GST_1 | DN34237_c0_g1 | Q9M0G0 | Glutathione hydrolase 3 | Vacuole | 4.6 | 1 | 4.6 |
| GT_1 | DN32482_c0_g1 | Q6VAA9 | Glycosyltransferase 73E1 UDP | Uncharacterized | 7 | 3.1 | 2.2 |
| GT_2 | DN30716_c0_g1 | Q9C768 | Glycosyltransferase 76B1 UDP | Intracellular membrane-bounded organelle | 4.9 | 2.1 | 2.3 |
| GT_3 | DN44583_c0_g1 | Q6VAB0 | Glycosyltransferase 85C2 UDP | Uncharacterized | 5.1 | 2.5 | 2 |
| GT_4 | DN30626_c0_g1 | Q9LZD8 | Glycosyltransferase 89A UDP | Intracellular membrane-bounded organelle | 8.7 | 1.8 | 4.8 |
| GT_5 | DN23238_c0_g1 | Q6VAA4 | Glycosyltransferase 85C1 UDP** | Uncharacterized | 13.4 | 6.8 | 2 |
Differentially expressed genes were selected using a p-value and FDR threshold set at ≤0.001; t0 = without glyphosate treatment; t1 = with glyphosate treatment—RNA was obtained from plants collected at several timepoints up to 288 h after treatment and pooled; GR: glyphosate-resistant biotype; GS: glyphosate-sensitive biotype. ** indicates that the gene was subjected to qRT-PCR analysis (See Figure 4).