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Isotype switching promoted by VLPs is independent of MAVS. Total IgG, IgG1, IgG2b and IgG2c were measured by ELISA 10 days after s.c immunisation with 50 μg of VLP in age and sex-matched MAVS knock-out and WT counterparts. VLP-specific antibodies. Mean + SEM n = 5. OD50 are titres defined as those dilutions that reached half the maximal OD observed for the assay.
