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. 2019 Jul 11;178(2):374–384.e15. doi: 10.1016/j.cell.2019.05.055

Figure 3.

Figure 3

Ceased Intercellular Communication after Ionophore Treatment Coincides with a Major Structural Rearrangement of the Septal Junction Cap

(A) Shown is a 13.5 nm-thick slice through a cryotomogram (magnified view in box) of the septal area of a CCCP-treated Anabaena filament. SJs are indicated by arrowheads. CM, cytoplasmic membrane; PG, septal peptidoglycan; TM, thylakoid membranes. Bar, 100 nm.

(B–E) Subtomogram averaging of SJs in the CCCP-treated non-communicating “closed” state (B–D) revealed major structural rearrangements in the cap module, compared to the “open” state (E). Shown are surface representations (B and C), and longitudinal and cross-sectional slices (0.68 nm) through the averages (D and E). Sliced positions are indicated in (D) and (E). Bars, 10 nm.

See also Figure S4 and Videos S4 and S5.