Table 3.
TPC, TFC, and antioxidant capacity of fractions separated from EtOAc extract of C. hindsii leaves.
| Fractions | Dilutions | TPC (mg GAE/ g Fraction) | TFC (mg RE/ g Fraction) | Antioxidant Activity | ||
|---|---|---|---|---|---|---|
| ABTS IC50 (µg/mL) | DPPH IC50 (µg/mL) | LPI (%) | ||||
| P1 | (C:M = 99:1) F1–20 | 19.76 ± 0.714 k | 40.06 ± 0.16 g | - | - | 58.88 |
| P2 | (C:M = 99:1) F20–40 | 15.36 ± 0.55 l | 29.08 ± 0.28 h | - | - | 58.01 |
| P3 | (C:M = 99:1) F40–60 | 111.19 ± 0.36 h | 41.65 ± 0.16 g | - | - | 59.84 |
| P4 | (C:M = 99:1) F60–80 | 84.52 ± 1.35 i | 41.11 ± 0.06 g | - | - | 57.74 |
| P5 | (C:M = 99:1) F80–100 | 168.93 ± 0.55 e | 93.36 ± 0.67 e | 124.57 ± 4.37 a | 241.53 ± 6.52 b c | 84.25 |
| P6 | (C:M = 99:1) F100–120 | 21.31 ± 0.21 k | 39.43 ± 0.16 g | - | - | 59.32 |
| P7 | (C:M = 97:3) F1–20 | 159.28 ± 1.09 f | 90.96 ± 0.11 e | 132.04 ± 3.43 a | 410.60 ± 21.70 a | 82.41 |
| P8 | (C:M = 95:5) F1–20 | 23.93 ± 0.74 j | 40.24 ± 0.21 g | - | - | 57.22 |
| P9 | (C:M = 9:1) F1–20 | 255.29 ± 0.55 c | 120.20 ± 0.16 c | 62.78 ± 15.55 c | 134.88 ± 2.56 d | 87.40 |
| P10 | (C:M = 8:2) F1–20 | 187.62 ± 0.95 d | 112.31 ± 0.16 d | 73.84 ± 5.22 b | 221.06 ± 11.86 c | 86.09 |
| P11 | (C:M = 7:3) F1–20 | 150.50 ± 0.00 g | 91.05 ± 0.37 e | 129.05 ± 3.09 a | 246.98 ± 11.20 b | 83.46 |
| P12 | (C:M = 5:5) F1–20 | 500.13 ± 0.55a | 138.17 ± 0.85 a | 26.57 ± 0.74 e | 78.08 ± 0.66 e | 89.76 |
| P13 | (C:M = 3:7) F1–20 | 374.64 ± 0.55 b | 127.43 ± 3.55 b | 41.83 ± 5.3 ed | 79.29 ± 1.06 e | 88.71 |
| P14 | (C:M = 1:9) F1–20 | 148.93 ± 0.55 g | 49.61 ± 0.76 f | 133.84 ± 1.46 a | 420.10 ± 22.50 a | 81.89 |
| BHT | Standard | n.d. | n.d. | 7.22 ± 0.89 f | 43.40 ± 3.52 f | 91.86 |
| MeOH | - | n.d. | n.d | n.d | n.d | 9.45 |
Data presented means ± standard deviations (SD). Different letter in a column indicated significantly different by Tukey’s test (p < 0.05); - = not detected; MeOH = methanol; BHT = dibutyl hydroxytoluene; positive control of antioxidant assay; TPC = total phenolic contents; TFC = total flavonoid contents; LPI = lipid peroxidation inhibition; GAE = gallic acid equivalent; RE = rutin equivalent; ABTS = 2,2-azinobis (3-ethylbenzothiazoline 6-sulfonic acid); DPPH = 2,2-diphenyl-1-picrylhydrazyl.