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. 2019 Jun 14;7(2):51. doi: 10.3390/vaccines7020051

Figure 2.

Figure 2

The gH peptide within the HA/gH chimeric viruses is conformationally similar to the native CMV gH protein. (A) Immunofluorescence staining of HEK293T cells transfected with plasmids encoding the HA/gH chimeras (HA/gH-7AA, HA/gH-10AA, and HA/gH-13AA), wild-type (wt) HA, and the full CMV gH protein were probed using anti-influenza A monoclonal antibody (KB2) and anti-CMV gH monoclonal antibody (10C10). Empty vector was utilized as a negative control. (B) MDCK cells infected with the chimeric HA/gH and wt-influenza viruses (MOI 0.25) were probed at 48 hpi with 10C10 and anti-influenza A polyclonal mouse serum (α-IAV sera). (C) Growth curves in MDCK cells of the chimera HA/gH viruses (HA/gH-7AA, HA/gH-10AA and HA/gH13AA) and of the wt-influenza virus (wt virus).