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. 2019 Jun 20;8(2):82. doi: 10.3390/pathogens8020082

Figure 1.

Figure 1

Mycoplasma mycoides titer in caprine and bovine precision cut lung slices (PCLS). Adherent bacteria to caprine (A) and bovine (C) PCLS were determined using qRT-PCR, while the number of non-adherent bacteria released to the media in caprine (B) and bovine (D) PCLS were determined via plating serial dilutions of culture supernatants on PH agar plates. Adherent Mmc GM12 increased in caprine PCLS (A) in time, however, a significant decrease in the number of non-adherent Mmm Afadé was observed in caprine PCLS (B). In bovine PCLS, adherent Mmc GM12 and Mmm Afadé showed an increase in number in a time-dependent manner (C) and the non-adherent bacteria remained constant throughout the infection period (D). Three biological replicates, error bars indicate SEM, **p-value < 0.01.