Figure 3.

Down-regulation of CDK11 inhibits clonal survival and tumorsphere formation in melanoma cells. A375 and WM1366 cells were transfected with 30 nM siRNAs as indicated in the legends and as described in materials and methods. (A) For clonal survival analysis, cells were plated onto 35 mm plates 48 h post-transfection and colonies were stained and counted seven days after plating. Left: The chart presents means ± SD from three experiments with three replicate plates each. ^ = p < 0.0001. Right: Representative crystal violet stained colonies on 35 mm plates. Cell lines are indicated to the left of images and siRNA transfections are indicated below plate images. (B) For tumorsphere formation, cells were plated into 96-well ultra-low attaching plates 48 h post-transfection and images captured 96 h after plating. Left: The chart presents means ± SD from three experiments with three areas each. ^ = p < 0.0001. Right: Representative tumorsphere images. Cell lines are indicated to the left of images and siRNA transfections are indicated below plate images.