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. 2019 Apr 12;4(2):63. doi: 10.3390/tropicalmed4020063

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Localization of envelope (E) protein in cerebral cortex of dengue virus (DV) serotype 2 infected mice. (A) Low magnification (10 ×) of mouse brain tissue from control group showed negative staining. (B) Positive E staining (pink) in cytoplasm of neuronal cells was revealed by anti-E specific monoclonal antibody (clone 4G2) and alkaline phosphatase secondary antibody at low magnification (10 ×). (C) A high magnification (20 ×) of B showed distribution of E protein in the majority of neuronal cells in the cerebral cortex region.