Figure 7.

(A) Low power of an infected microglial cell showing typical irregular contour-shaped cell with dark nucleus with distinct peripheral heterochromatin condensation. Cytoplasmic area along the right of the nucleus shows dilated ER and collections of vesicles (inset). (B) Higher power of the perinuclear region showed a dilated ER (between black arrows) that courses along the nucleus, filled with dengue virions. The cytoplasm contains virus filled vesicles (black arrow heads) mixed with aggregates of double-membrane bodies (white arrowhead) and a group of swollen mitochondria (M) flanking the vesicles. (C) High power view of B inset (area within the rectangle) shows ribosome-studded ER containing dense core virions with a diameter of 40–50 nm. The core density and sizes of some of the intravesicular virions vary. Vesicular packets with round and oblong/tubular vesicles were observed (white arrow).