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The expression of chCH25H is upregulated by ALV-J infection and IFN-α treatment in DF1 cells. (A) Heatmap of 152 chicken anti-ALV-J ISG candidates. (B) The expression of chCH25H was significantly increased in DF1 cells after treatment with 1000 U/mL IFN-α 24 h and 48 h. (C) DF1 cells were infected with ALV-J strain SCAU-HN06. chCH25H expression was significantly increased at 24 and 48 hpi. * p < 0.05; ** p < 0.01.
