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. 2019 May 30;11(6):498. doi: 10.3390/v11060498

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of the core promoter of chCH25H. (A) The first nucleotide of chCH25H coding region is numbered −1, and the different dual luciferase reporter vectors are named PGL3- p2077(−2077/−1), p1000 (−1000/−1), p500 (−500/−1), p250 (−250/−1), p125 (−125/−1) and p75 (−75/−1), respectively. (B) Dual luciferase assay showed that the inserted sequences of 75 bp, 125 bp, 250 bp, 500 bp, 1000 bp and 2077 bp in presumed promoter region all significantly increased the relative luciferase activity compared to the empty pGL3-basic vector in DF1 cells. (C) Screening of GAS and ISRE elements in the upstream region of chCH25H. ** p < 0.01; *** p < 0.001.