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. 2019 May 30;11(6):498. doi: 10.3390/v11060498

Figure 5.

Figure 5

Overexpression of chCH25H inhibits ALV-J replication. (A) Exogenous CH25H and EGFP genes was expressed in DF1 cells at 24 h post transfection. (B) The expression of SCAU-HN06 gp85 gene was detected by qPCR in DF1 cells at 24 and 48 hpi. (C) The expression of ALV-J p27 protein was detected by ELISA in DF1 cells at 24 and 48 hpi. (D) Expression levels of the ALV-J envelope protein in DF1 cells were detected by Western blotting using mouse antibody JE9. “CH25H” on the graph represents overexpression of CH25H in DF1 cells. “EGFP” represents control. ** p < 0.01.