Effects of intranasal pseudorabies virus (PRV) AH02LA strain on the mRNA expression of genes related to cytokines and barrier function in the ileal and colonic mucosa of piglets at day 5 post intranasal infection. The values are expressed as the means ± SEM (n = 5). Asterisks indicate statistically significant difference from control. * p < 0.05, ** p < 0.01. The β-actin mRNA level was used to normalize the relative amount of each studied mRNA, and the2−ΔΔCt method was used to analyze the data. IL, interleukin; IFN-γ, interferon-γ; TNF-α, tumor necrosis factor-α; ZO-1, zonula occludens-1.