Table 3.
GFP expression efficiency of Hansen 536 MB slices after three and 12 weeks of culture on WPMm5 medium supplemented with different kanamycin concentrations, or without kanamycin selection.
| Kan Concentration (μM) | Number of Weeks after Co-Culture | Mean Number of GFP Spot/Explant ± SE | GFP MB (%) ± SE * | Number of MB Slices with Lzs | GFP MB Lzs (%) ± SE ** |
|---|---|---|---|---|---|
| 0 | 3 | 1.17 ± 0.37 (b) | 26.67 ± 5.75 (c) | 12 | 20 ± 5.21 (ns) |
| 12 | 0.82 ± 0.38 (A) | 6.67 ± 3.25 (A) | 2 | 3.33 ± 2.34 (NS) | |
| 17 | 3 | 2.10 ± 0.33 (ab) | 63.33 ± 6.5 (a) | 15 | 25 ± 5.64 (ns) |
| 12 | 0.78 ± 0.29 (A) | 13.33 ± 4.42 (A) | 4 | 6.67 ± 3.25 (NS) | |
| 42 | 3 | 3.17 ± 0.24 (a) | 48.33 ± 6.27 (b) | 22 | 36.67 ± 6.27 (ns) |
| 12 | 0.68 ± 0.36 (A) | 8.33 ± 3.60 (A) | 1 | 1.67 ± 1.67 (NS) |
* GFP MB (%), Percentage of MB slices showing GFP fluorescence, selected either by kanamycin and GFP screening or by GFP visual selection alone (0 μM Kan), after three and 12 weeks on culture media. ** GFP MB Lzs (%), Percentage of MB slices showing large zones of GFP expression per total number of Agrobacterium inoculated MB slices. Values followed by small letters (a, b, c) compare explants cultured at three weeks after co-culture, while capital letter (A, B, C) compare explants cultured at 12 weeks after co-culture. Values are means (n = 60) ± standard error (SE). Means in columns with different letters are significantly different according to Duncan test (p < 0.05). NS, not significant.