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. 2019 May 28;11(6):487. doi: 10.3390/v11060487

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Standard curves generated by binding HIVIG to plates coated with recombinant gp120, recombinant gp120 coated CEM (cCEM) cells and to HIV-infected CEM (iCEM) cells. Binding of a 2-fold serial dilution of HIVIG to ELISA plates coated with rgp120 (a). Binding of a 3-fold serial dilution of HIVIG to cCEM (b) and iCEM (c) and their CFSE⁺ CEM cell internal controls. The y-axis shows the optical density measured at 450 nm (OD450nm) generated by HIVIG binding to rgp120 coated plates (a). In (b) and (c), the y-axes show the mean fluorescence intensity (MFI) generated by HIVIG binding to (b) cCEM (closed circles) and CEM (closed squares) and (c) to iCEM (closed circles) and CEM (closed squares). The standard curve in (b) shows average values for 5 replicates; the curve in (c) is shows average values for 8 replicates. Each point and its error bars represent averages and standard deviations for these values. OD450nm = optical density at a wave length of 450 nanometers; MFI = mean fluorescence intensity; CEM = CEM.NKr.CCR5 cell line.