Figure 5.

Correlations between rgp120/HIV Envelope-specific antibody levels quantified by a plate-based ELISA and two flow-cytometry based assays. Spearman correlation tests were used to evaluate the significance of the correlation between results generated by the (a) plate-based ELISA and flow cytometry-based quantification assays using iCEM as target cells, (b) the two flow cytometry-based quantification assays using iCEM and cCEM cells as target cells, and (c) the plate-based ELISA assay and the flow cytometry-based quantification assays using cCEM as target cells for all HIV-infected subjects (a–c) or for untreated progressors, treated progressors and elite controllers separately (d). Values in (d) indicate the correlation coefficient “r” for each comparison. The color scale indicates the “p” values for each correlation. PBE = plate-based ELISA; cCEM = recombinant gp120 coated CEM cells; iCEM = HIV-infected CEM cells; UTP = untreated progressors; TP = treated progressors; EC = elite controllers.