Table 1. Merlin-proximal proteins.
The proteins that met the selection criteria from proximity biotinylation assays with Merlin-BirAR118G are arranged by the average number of MS peptide reads normalized to protein molecular weight, along with the number of peptides from these proteins identified in all other samples. n = 3 independent experiments. Merlin and known Merlin-binding proteins Angiomotin (Amot), angiomotin-like protein 1 (Amotll), Erbin (Erbb2ip), and moesin (Msn) are highlighted in bold.
| Gene | Description | MW | Ctrl | WT | BM | FH | AR | 6N |
|---|---|---|---|---|---|---|---|---|
| Nf2 | Neurofibromin 2 (Merlin) | 69,776 | 4.7 | 187.3 | 81.6 | 220.8 | 241.7 | 124.7 |
| Rai14 | Retinoic acid induced 14 | 108,852 | 11.2 | 80 | 19.2 | 49.3 | 61 | 26.3 |
| Epb41l2 | Erythrocyte membrane protein band 4.1-like 2 | 109,940 | 10.9 | 48.7 | 18.5 | 81 | 54.7 | 19.4 |
| Utrn | Utrophin | 392,706 | 13.9 | 101.5 | 12.1 | 137.8 | 100 | 23.7 |
| Mllt4 | Afadin | 206,499 | 1.9 | 48.3 | 5.9 | 48.1 | 29.3 | 6.8 |
| Amotl1 | Angiomotin-like protein 1 | 107,950 | 0.6 | 24.4 | 9.1 | 3.6 | 26.9 | 8.5 |
| Ruvbl1 | RuvB-like AAA ATPase 1 | 50,214 | 2.2 | 11.2 | 1 | 9 | 14.6 | 14 |
| Kank2 | KN motif and ankyrin repeat domains 2 | 90,245 | 4.4 | 19.6 | 3.5 | 21.2 | 16.6 | 9.1 |
| Epb41l3 | Erythrocyte membrane protein band 4.1-like 3 | 103,338 | 2.8 | 20.3 | 5.4 | 33.3 | 29.4 | 7.9 |
| Epb41l1 | Erythrocyte membrane protein band 4.1-like 1 | 98,315 | 0 | 19.3 | 7.3 | 18.7 | 14.6 | 2.2 |
| Tjp1 | ZO-1, tight junction protein 1 | 194,742 | 3.4 | 37.3 | 6.5 | 21.4 | 26 | 5.1 |
| Tp53bp2 | ASPP2, tumor protein p53 binding protein 2 | 125,301 | 1.8 | 21.5 | 0.4 | 13.3 | 17.8 | 2.9 |
| Rassf8 | Ras association domain family member 8 | 48,103 | 0 | 8.2 | 0.8 | 5.8 | 9.9 | 1.7 |
| Vcl | Vinculin | 116,717 | 1.2 | 17.1 | 1.2 | 6.5 | 16.9 | 8 |
| Prune | Prune exopolyphosphatase | 50,239 | 0 | 7 | 1.7 | 2.4 | 6.4 | 2.9 |
| Mprip | Myosin phosphatase Rho-interacting protein | 116,408 | 2.5 | 16.2 | 2.4 | 24.4 | 22.8 | 5 |
| Erc1 | ELKS/RAB6-interacting/CAST family member 1 | 128,331 | 3.1 | 17.8 | 1.7 | 14.2 | 26 | 10.3 |
| Amot | Angiomotin | 120,915 | 0 | 16.2 | 2 | 0 | 14.4 | 4.3 |
| Phldb2 | Pleckstrin homology-like domain, family B member 2 | 141,486 | 1.3 | 18.9 | 3.4 | 12.2 | 14 | 8.1 |
| Dlg5 | Discs, large homolog 5 | 214,386 | 0 | 28.5 | 0 | 5 | 26.9 | 6.4 |
| Erbb2ip | Erbb2-interacting protein | 157,248 | 0 | 19.3 | 3.4 | 23.6 | 14.9 | 0.5 |
| Myh10 | Myosin heavy chain 10, nonmuscle | 228,996 | 7.5 | 25.6 | 43.4 | 9.9 | 21.1 | 35 |
| Lpp | Lipoma-preferred partner | 65,891 | 1 | 7.1 | 2.1 | 6.1 | 11.7 | 6.6 |
| Cobll1 | Cordon-bleu WH2 repeat protein-like 1 | 137,382 | 0.6 | 14.8 | 0.4 | 23.7 | 8.9 | 1.6 |
| Stk38 | Serine/threonine kinase 38 | 54,174 | 0.3 | 5.7 | 1.1 | 4.5 | 4.4 | 3.9 |
| Msn | Moesin | 54,174 | 0 | 5.7 | 3.5 | 7.9 | 15.2 | 6.5 |
| Cep55 | Centrosomal protein of 55 kDa | 53,930 | 0 | 5.3 | 0.2 | 0 | 1.8 | 0 |
| Actn1 | Actinin, a1 | 103,068 | 0 | 10.1 | 4.3 | 0.4 | 12 | 5.2 |
| Scrib | Scribbled planar cell polarity protein | 174,059 | 0.6 | 16.4 | 2.5 | 15.6 | 16.7 | 3.8 |
| Dlg1 | Discs, large homolog 1 | 100,120 | 0 | 9.3 | 1.5 | 8.6 | 12.8 | 0 |
| Phldb1 | Pleckstrin homology-like domain, family B, member 1 | 150,070 | 0.6 | 13.8 | 1.2 | 18.3 | 11.3 | 1.4 |
| Shroom4 | Shroom family member 4 | 163,238 | 1.8 | 14.8 | 1.2 | 19.3 | 8.6 | 2.1 |
| Ptpn13 | Protein tyrosine phosphatase, type 13 | 270,334 | 1.3 | 24.4 | 7.8 | 23.8 | 22 | 5.1 |
| Mpdz | Multiple PDZ domain protein | 218,711 | 0 | 19.2 | 0.7 | 2.4 | 19.6 | 2.7 |
| Tnks1bp1 | Tankyrase 1 binding protein 1, 182 kDa | 181,825 | 1.5 | 14.9 | 2.6 | 31.3 | 19.8 | 7 |
| Tjp2 | Tight junction protein 2 | 131,280 | 0.3 | 10.6 | 1.2 | 4 | 3.9 | 3.2 |
| Phactr4 | Phosphatase and actin regulator 4 | 76,632 | 0 | 5.8 | 0.6 | 6.1 | 4 | 0.5 |
| Fam129b | Family with sequence similarity 129, member B | 84,819 | 0 | 6 | 3.7 | 2.6 | 6 | 2.5 |
| Ctnnd1 | Catenin (cadherin-associated protein), δ1 | 104,925 | 0 | 7.3 | 1.6 | 5.8 | 5.3 | 1.4 |
| Peak1 | Pseudopodium-enriched atypical kinase 1 | 191,097 | 0.6 | 12.7 | 5.6 | 17.4 | 13.1 | 4.8 |
| Tln2 | Talin 2 | 253,621 | 0.3 | 15.1 | 0 | 7.6 | 8.3 | 0.5 |
| Triobp | TRIO and F-actin binding protein | 223,368 | 0.9 | 13.2 | 2 | 16.4 | 11.6 | 1.6 |
| Sipa1 | Signal-induced proliferation-associated 1 | 112,066 | 0.6 | 6 | 0.4 | 4.5 | 8 | 3.5 |
| Sipa1l1 | Signal-induced proliferation-associated 1-like 1 | 197,031 | 0 | 9.9 | 1.9 | 4.1 | 6 | 0.9 |
| Magi3 | Membrane associated guanylate kinase 3 | 161,672 | 0 | 6.7 | 2.3 | 0 | 3.5 | 0 |
| Ehbp1l1 | EH domain binding protein 1-like 1 | 184,834 | 0 | 7.4 | 0 | 19.9 | 7.8 | 1.6 |
| Cgn | Cingulin | 136,447 | 0 | 5.3 | 0 | 0 | 2.3 | 0 |
| Rrbp1 | Ribosome binding protein 1 | 172,879 | 0.6 | 5.7 | 11.7 | 2.2 | 10.1 | 5.8 |
| Magi1 | Membrane associated guanylate kinase 1 | 161,974 | 0 | 5.3 | 3.8 | 1.6 | 2 | 0 |
| Atg2b | Autophagy-related 2B | 231,399 | 0 | 7.1 | 1.4 | 2.4 | 3.5 | 5.5 |
| Itsnl | Intersectin 1 | 194,297 | 0 | 5.6 | 0 | 8.5 | 5.1 | 0 |
| Speg | SPEG complex locus | 354,343 | 0.9 | 8.7 | 19.1 | 11.2 | 13.4 | 1 |
| Trio | Trio Rho guanine nucleotide exchange factor | 347,861 | 0.6 | 6.3 | 1.4 | 9.6 | 5 | 0.7 |