Table 2.

Recovery of proteins that constitute the NM protein corona—both the fraction in solution and the fraction remaining on the NM post extraction.

	Experimental Component	TiO2	TiO2-PVP	TiO2-Dispex	PS	PS-COOH
On-particle digest	Summed MS signal intensity	1.0 × 1011	1.1 × 1010	7.7 × 1010	8.9 × 109	3.6 × 1010
	High confidence proteins	80	81	83	77	86
Remaining in-gel	Summed MS signal intensity	5.1 × 107	3.3 × 106	1.2 × 108	1.4 × 107	2.6 × 108
	% summed MS signal remaining	0.05%	0.03%	0.16%	0.15%	0.73%
	High confidence proteins	0	0	0	2	3
	Median remaining on particle *	0.05%	0.10%	0.09%	0.15%	0.80%

* calculated as the median of individual protein abundance ratios using peaks of the top 3 peptide intensities (Remaining on-particle/On-particle digestion).