Figure 2.

Behavior endpoint of control and Fe₃O₄ MNPs-exposed zebrafish in novel tank exploration test after two weeks’ exposure. (A) Average speed, (B) freezing time movement ratio, (C) time in top duration, (D) number of entries to the top, (E) latency to enter the top, and (F) total distance traveled in the top were analyzed. (G–I) The locomotion trajectories of control as well as 1 and 10 ppm MNPs-exposed fish in the novel tank test. The black line represents the control group, the red line represents the low concentration MNPs group (1 ppm), and the blue line represents the high concentration MNPs group (10 ppm). The data are expressed as the median with interquartile range and were analyzed by two-way ANOVA with Geisser-Greenhouse correction. To observe the main column (Fe₃O₄ MNPs) effect, Dunnett’s multiple comparison test was carried out. (n = 20, * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001).