Figure 6.

Behavior endpoint comparisons between the control group, 1 ppm, and 10 ppm Fe₃O₄ MNPs-exposed zebrafish groups after two weeks of exposure. (A) Interaction time percentage, (B) longest duration in the separator side, (C) average speed, and (D) average distance to the separator were analyzed. (E–G) The locomotion trajectories for the control, 1, and 10 ppm MNPs-exposed fish in the conspecific social interaction test. The data are expressed as the median with an interquartile range and were analyzed by the Kruskal-Wallis test, which continued with Dunn’s multiple comparisons test as a follow-up test (n = 20, * p < 0.05, ** p < 0.01).