(A) Schematic diagram of experimental design. Hereinafter, the progeny from dysgenic/invasive cross are referred to as “Invaded”; the P-element-silenced offspring from noninvasive (reciprocal) cross are shortened as “Protected”.
(B) Ovarian morphology and fertility of invaded and protected progeny. Invaded flies have normal size ovaries at 18°C, but rudimentary ovaries at 25°C.
(C) Genome sequencing to globally probe new transposition events in invaded and protected progeny. Each dot represents one transposon family.
(D) Small RNA sequencing to detect the production of piRNAs mapped to transposon. Each dot represents one transposon family. Only piRNAs targeting P-elements (green dots) decreased in invaded progeny, compared with protected controls.
See also Figure S1.