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. 2019 Jul 15;12:408. doi: 10.1186/s13104-019-4463-1

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Optimization of the LAMP assay. a The LAMP assay was performed using the LAMP primer set with or without a loop primer. b The LAMP reaction was performed at 61, 63, 65, and 67 °C. Amplification profiles were obtained from positive control (PC) samples using Aconitum. japonicum subsp. subcuneatum DNA (5 ng) and negative control (NC) samples using Anemone flaccida DNA (5 ng)