Table 1.
Results of LAMP detection assay
| Sample | DNA template | Reaction timea | |
|---|---|---|---|
| Sensitivity | Ac. japonicum subsp. subcuneatum | 10 ng | 6.8 ± 0.2 min |
| 1 ng | 8.0 ± 0.3 min | ||
| 100 pg | 8.9 ± 0.2 min | ||
| 10 pg | 10.3 ± 0.4 min | ||
| 1 pg | 11.9 ± 0.3 min | ||
| 100 fg | nd | ||
| (No template) | nd | ||
| Specificity | Ac. japonicum subsp. subcuneatum | 5 ng | 7.1 ± 0.1 min |
| Ac. sachalinense subsp. yezoense | 5 ng | 7.3 ± 0.0 min | |
| Ac. chinense | 5 ng | 7.2 ± 0.2 min | |
| Anemone flaccida | 5 ng | nd | |
| Parasenecio delphiniifolius | 5 ng | nd | |
| Mixture | Ac. japonicum subsp. subcuneatum | 50 pg | 10.0 ± 0.2 min |
| Ac. japonicum subsp. subcuneatum + Anemone flaccida | 50 pg + 50 ng | 9.8 ± 0.3 min | |
| Anemone flaccida | 50 ng | nd |
nd not detected
aThe reactions were carried out in triplacates