Figure 2.

Time course of 1a–d, 1f, and 1h–k hydrolysis by human recombinant monoacylglycerol lipase (hMAGL). 25ng/well of hMAGL (circles) or buffer alone (empty squares) were incubated in 96-well black plate, at room temperature, with 5 μM of each compound in total volume of 100 μL/well, as described in the experimental section. Fluorescence was measured at indicated time points with a Jasco FP-8300 fluorometer (Jasco Europe, Cremella (LC) Italy) using the kinetic mode (λ_ex = 571 nm, λ_em = 588 nm, slit = 5.0). Data are mean ± standard error of independent experiments.