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Action of hMAGL on different concentrations of 1a–d, 1f, 1h–j, in the presence of DMSO (10%, v/v). The reactions were conducted in 50 mM Tris-HCl buffer (pH 7.4, 1 mM EDTA) with 25 ng/well of hMAGL. Values are the means of triplicates ± standard error. The kinetic parameters Km and Vmax were determined via computer-assisted nonlinear regression analysis using GraphPad Prism 6.0c (GraphPad Software, San Diego, CA 92108).
