Figure 5.
miRNAs (miR-1273f, miR-1184, and miR-198) may interact with the predicted target sites in the 3′ UTR of IFN-β mRNA. (A) Predicted target sites for miR-1273f, miR-1184, and miR-198 in IFN-β 3′ UTR mRNA were shown. Nucleotide positions in the 3′ UTR, which were complementary to the miR-1273f, miR-1184, and miR-198 seed region, were indicated. (B) Effect of miR-1273f, miR-1184, and miR-198 mimics on the luciferase expression of reporter constructs containing the putative miRNA binding sites from the IFN-β 3′ UTR was shown. The values were expressed as the mean of the results from three independent experiments, with the error bar showing standard deviations. Statistical significance is indicated: * p < 0.05.