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. 2019 Jun 6;11(6):527. doi: 10.3390/v11060527

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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MicroRNAs benefit NDV replication. (A) Nucleocapsid protein (NP) mRNA was detected with RT-qPCR in HeLa cells transfected with miRNA mimics and then infected with NDV. β-Actin was an internal control. (B) NP protein was detected by Western blot in HeLa cells transfected with miRNA mimics and then infected with NDV. β-actin was an internal control. (C) The integrated density of NP expression was analyzed in (B) with Image J software 1.8.0. β-actin was an internal control. Error bars are the SEM from three independent experiments. Statistical significance is indicated: ** p < 0.01 and * p < 0.05.