Table 2.
miRNA Name | miRNA Sequence a (5′-3′) | miRbase Accession No. | RT Primer Sequence (5′-3′) | Forward PCR Primer Sequence (5′-3′) |
---|---|---|---|---|
hsa-miR-1273f | GGAGAUGGAGGUUGCAGUG | MIMAT0020601 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCACTGC | CGCGCGGGAGATGGAGGTT |
hsa-miR-1184 | CCUGCAGCGACUUGAUGGCUUCC | MIMAT0005829 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGAAGC | GCGCCTGCAGCGACTTGATG |
hsa-miR-198 | GGUCCAGAGGGGAGAUAGGUUC | MIMAT0000228 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGAACCT | TGCGGGTCCAGAGGGGAGAT |
a The reverse PCR primer of various miRNAs is universal, and the sequence is 5′-ATCCAGTGCAGGGTCCGAGG-3′.