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. 2019 Jun 6;11(6):527. doi: 10.3390/v11060527

Table 2.

Specific miRNA primers for RT-qPCR.

miRNA Name miRNA Sequence a (5′-3′) miRbase Accession No. RT Primer Sequence (5′-3′) Forward PCR Primer Sequence (5′-3′)
hsa-miR-1273f GGAGAUGGAGGUUGCAGUG MIMAT0020601 GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCACTGC CGCGCGGGAGATGGAGGTT
hsa-miR-1184 CCUGCAGCGACUUGAUGGCUUCC MIMAT0005829 GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGAAGC GCGCCTGCAGCGACTTGATG
hsa-miR-198 GGUCCAGAGGGGAGAUAGGUUC MIMAT0000228 GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGAACCT TGCGGGTCCAGAGGGGAGAT

a The reverse PCR primer of various miRNAs is universal, and the sequence is 5′-ATCCAGTGCAGGGTCCGAGG-3′.