Figure 3.
Action of chlorprothixene on NMDAR currents in isolated Wistar rat hippocampal CA1 pyramidal neurons. (A) Concentration dependence of action of chlorprothixene at −80 mV holding voltage in the absence of magnesium in extracellular solution was fitted by Hill equation. NMDAR currents were induced by 100 μM NMDA + 10 μM glycine. IC50 = 2.5 ± 0.6 µM and Hill coefficient = 1.3 ± 0.2. (B) Washout kinetics of chlorprothixene in the presence of agonists, weighted time constant from double-exponential fitting τ = 35 ± 11 s. (C) Voltage dependence of action of chlorprothixene fitted by Woodhull model. Δ = 0.9 ± 0.2, suggesting a binding site deep in the channel pore. (D) Chlorprothixene demonstrates partial trapping in the double-pulse protocol at −80 mV holding voltage.